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ScienCell primary htm cells
Effects of Lentiviral Vector-Mediated C3 Expression on <t>HTM</t> <t>Cells</t>
Primary Htm Cells, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary htm cells  (DuPont de Nemours)
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DuPont de Nemours primary htm cells
(A) <t>HTM</t> <t>cells</t> stained for YAP (red) and counterstained with DAPI (blue) to identify the nuclei. YAP localization in HTM cells is mixed between nuclear and cytoplasmic. Nuclear localization is more pronounced on TCP (> 1 GPa) than on the 5 kPa hydrogel. With Lat-B treatment, nuclear localization is decreased on the 5 kPa hydrogel but increased on TCP. Scale bar is 10 μm. (B) These observations can be quantified through nuclear contrast (NC) measurements, revealing a significant decrease of NC on 5 and 75 kPa hydrogels with Lat-B, while NC significantly increases on TCP. * indicates p<0.05, Mann-Whitney U test. (C) Lat-B increases phosphorylation of YAP on 5 and 75 kPa hydrogels, while decreasing it on TCP. (D) Optical Density (O.D.) values for immunoblots of phosphorylated YAP, total YAP, and TAZ.
Primary Htm Cells, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+htm+cells/pmc03737383-47-27-30?v=DuPont+de+Nemours
Average 90 stars, based on 1 article reviews
primary htm cells - by Bioz Stars, 2026-08
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Effects of Lentiviral Vector-Mediated C3 Expression on HTM Cells

Journal: Molecular Therapy

Article Title: Lentiviral Vector-Mediated Expression of Exoenzyme C3 Transferase Lowers Intraocular Pressure in Monkeys

doi: 10.1016/j.ymthe.2019.04.021

Figure Lengend Snippet: Effects of Lentiviral Vector-Mediated C3 Expression on HTM Cells

Article Snippet: Primary HTM cells (ScienCell Research Laboratories, Carlsbad, CA, USA; see https://www.sciencellonline.com for details) were cultured in TM Cell Medium (TMCM; ScienCell Research Laboratories) consisting of basal medium, 2% fetal bovine serum, 1% TM cell growth supplement (TMCGS), and 1% penicillin/streptomycin solution, at 37°C in an atmosphere of 5% CO 2 , as previously described.

Techniques: Plasmid Preparation, Expressing

(A) HTM cells stained for YAP (red) and counterstained with DAPI (blue) to identify the nuclei. YAP localization in HTM cells is mixed between nuclear and cytoplasmic. Nuclear localization is more pronounced on TCP (> 1 GPa) than on the 5 kPa hydrogel. With Lat-B treatment, nuclear localization is decreased on the 5 kPa hydrogel but increased on TCP. Scale bar is 10 μm. (B) These observations can be quantified through nuclear contrast (NC) measurements, revealing a significant decrease of NC on 5 and 75 kPa hydrogels with Lat-B, while NC significantly increases on TCP. * indicates p<0.05, Mann-Whitney U test. (C) Lat-B increases phosphorylation of YAP on 5 and 75 kPa hydrogels, while decreasing it on TCP. (D) Optical Density (O.D.) values for immunoblots of phosphorylated YAP, total YAP, and TAZ.

Journal: Experimental eye research

Article Title: Substratum Stiffness and Latrunculin B Modulate the Gene Expression of the Mechanotransducers YAP and TAZ in Human Trabecular Meshwork Cells

doi: 10.1016/j.exer.2013.05.014

Figure Lengend Snippet: (A) HTM cells stained for YAP (red) and counterstained with DAPI (blue) to identify the nuclei. YAP localization in HTM cells is mixed between nuclear and cytoplasmic. Nuclear localization is more pronounced on TCP (> 1 GPa) than on the 5 kPa hydrogel. With Lat-B treatment, nuclear localization is decreased on the 5 kPa hydrogel but increased on TCP. Scale bar is 10 μm. (B) These observations can be quantified through nuclear contrast (NC) measurements, revealing a significant decrease of NC on 5 and 75 kPa hydrogels with Lat-B, while NC significantly increases on TCP. * indicates p<0.05, Mann-Whitney U test. (C) Lat-B increases phosphorylation of YAP on 5 and 75 kPa hydrogels, while decreasing it on TCP. (D) Optical Density (O.D.) values for immunoblots of phosphorylated YAP, total YAP, and TAZ.

Article Snippet: Due to the known variability in YAP localization exhibited by different cell lines ( Miller et al., 2012 ), we propose to investigate these changes in primary HTM cells ( Dupont et al., 2011 ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window caption a7 caption a8 Schematic map of the YAP/TAZ pathway and its role in glaucoma YAP and TAZ can be localized to either the nucleus or cytoplasm.

Techniques: Staining, MANN-WHITNEY, Phospho-proteomics, Western Blot